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ناشرِ شانِ نبیؐ (نظم)

ناشرِ شانِ نبیؐ
جو ہمارے دل کو خوب اچھی طرح سے یاد ہیں ، ڈاکٹر شہزاد ہیں
جن کو حاصل نعت شہ میں قیمتی اسناد ہیں ، ڈاکٹر شہزاد ہیں

کام ان کا رات دن ترویجِ فکر نعت ہے ، خوب ان کی بات ہے
ناشرِ شانِ نبیؐ جو ماحیٔ الحاد ہیں ، ڈاکٹر شہزاد ہیں

قدر کرتا ہے زمانہ مانتے ہیںسب انھیں، سب کو ہی اچھے لگیں
فضل حق سے جو یکے از صاحبانِ داد ہیں ، ڈاکٹر شہزاد ہیں

جی نہیںلگتا فضائے غیر میںان کا کہیں، جس قدر ہو دل نشیں
نعت کی دنیا میں جو ہر آن خوش ہیں ، ڈاکٹر شہزاد ہیں

حمد و نعت و منقبت پر ان کی ہے گہری نظر ، ہر حوالہ معتبر
اک مفید انسان ہیں جو بہتریں اُستاد ہیں ، ڈاکٹر شہزاد ہیں

زینت علم و ادب ہیں حسنِ بزم از کیا، باوفا و بے ریا
حب آقاؐ میں جو فکر دہر سے آزاد ہیں ، ڈاکٹر شہزاد ہیں

معترف عارفؔ ہے ان کی ذات با کردار کا ، ہستی شہ کار کا
ہیں کراچی میں مگر طیبہ میں جو آباد ہیں ، ڈاکٹر شہزاد ہیں
محمد عارف قادری ۔واہ کینٹ

تمثیل الانبیاء (انبیا پر فلم سازی) اور اسلامی شریعت کا نقطہ نظر

All the Prophets have been respectable in the scriptures of all the world religioins. They were sent to this world for a significant cause and remained model role for human beings, especially in Islamic point of view they have been a part of belief; and any disgrace towards them is regarded an act of pegamism. In modern ages the concept of presenting the lives of the prophets in film stories is developing though it is a source of knowledge but for many reasons it held a sign of interrogation wich has been discussed in this Article.

Development of Recombinant Proteins for the Control and Diagnosis of Hydropericardium Syndrome

Hydropericardium syndrome (HPS) is a viral disease of poultry which is caused by Fowl adenovirus (FAdV). This virus belongs to family Adenoviridae and genus Aviadenovirus. In recent years, Hydropericardium syndrome (HPS) has emerged as one of the important diseases occurring in Pakistan and has caused heavy economic loss. Efforts have been made to develop conventional vaccines against this disease. These vaccines were formulated from infected liver homogenate. Unfortunately, formalin-inactivated liver organ vaccines failed to protect the poultry industry in the country. Hence, there is a need to develop a suitable vaccine to combat this disease. Currently, recombinant vaccine candidates are being developed for the prevention and control of some infectious diseases in several laboratories elsewhere. The present work is an effort to develop a recombinant protein, using molecular biology, biotechnological and immunological approaches for effective control and diagnosis of HPS. In the present study, the viral particle was isolated from natural outbreak of Hydropericardium syndrome in broilers, Punjab province of Pakistan using conventional methods. The existence of the virus was initially observed by Scanning Electron Microscopic examination. Icosahedral shaped viral particles of 70 – 80 nm in diameter were observed. Further, the presence of FAdV was confirmed by Polymerase Chain Reaction (PCR) by amplification of 730 bp variable region (L1 and part of P1 loop) of hexon gene. DNA sequence analysis and Phylogenetic analysis of the PCR product revealed that isolate is closely related with Indian fowl adenovirus – 4 isolate. To investigate which gene product encoded by fowl adenovirus plays vital role in immune response against the disease, two genes representing structural proteins of the virus (Penton base & short fiber) and one gene representing non structural protein (100K) were selected to develop recombinant constructs. To achieve this, the Penton base (1587bp), Short fiber (1437bp) and 100K (2397bp) genes were amplified by PCR and cloned in an expression vector (pET28a). The histidine residues along with thrombin protease site were engineered upstream to inserts (viral genes). The presence of recombinant DNA fragments were confirmed by double digestion method, PCR amplification of insert using gene specific primers and DNA sequencing of the inserts. Nucleotide sequences of inserts revealed that two genes (Penton base and Short fiber) of local isolate have >98% homology with the Indian FAdV-4 isolates, while one gene (100K) has 96% homology with the Russian FAdV-10 isolate. The recombinant constructs were expressed in E. coli. The expression of recombinant proteins was assessed by SDS-PAGE. Western blot analysis confirmed the presence of histidine tagged recombinant proteins i.e. short fiber (60Kda), penton base (65Kda) and 100K (95Kda) using anti His tag antibody. The three recombinant proteins were purified by Nickle affinity chromatography. The biological and immunological activity of recombinant proteins were assessed for potential use as antigen in vaccine and diagnostic (ELISA). The purified recombinant proteins were adjuvanted separately with Freund’s complete adjuvant and broilers were immunized. ELISA test was performed and antibody titers were determined against the respective recombinant proteins. The results indicated that protein constructs pSMJ-2 (penton base) and pSMJ-3 (short fiber) are more immunogenic antigens as compared to protein construct pSMJ-1 (100K) and commercial vaccine. Challenge protection test also proved that penton base (pSMJ-2) and short fiber (pSMJ- 3) protein constructs conferred 90% and 80% protection respectively against pathogenic virus challenge. Whereas 100K (pSMJ-1) protein construct and commercial inactivated vaccine provided 50% and 70% protection respectively. The results obtained by ELISA and challenge test in this study indicated that the constructed recombinant proteins are suitable candidates to develop subunit vaccine and diagnostic kit (strip test) thereby can be used for prevention and control of this disease.
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